In enzymology, a N-acylglucosamine 2-epimerase () is an enzyme that catalyzes the chemical reaction
Hence, this enzyme has one substrate, N-acyl-D-glucosamine, and one product, N-acyl-D-mannosamine.
This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is N-acyl-D-glucosamine 2-epimerase. Other names in common use include acylglucosamine 2-epimerase, and N-acetylglucosamine 2-epimerase. This enzyme participates in aminosugars metabolism. It employs one cofactor, ATP.
As of late 2019, three structures have been solved for this class of enzymes, with the PDB accession codes , , and . They show that the N-acylglucosamine 2-epimerase monomer folds as a barrel composed of ñ-helices, in a manner known as (ñ/ñ)<sub>6</sub>-barrel. The structures are presented as dimers, with the structures from Sus scrofa and Anabaena sp. CH1 having a different organization than the structure from Nostoc sp. KJV10.