In enzymology, a N-acyl-D-aspartate deacylase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are N-acyl-D-aspartate and H<sub>2</sub>O, whereas its two products are carboxylate and D-aspartate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-D-aspartate amidohydrolase. It employs one cofactor, zinc.