In enzymology, a N-acyl-<small>D</small>-amino-acid deacylase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are N-acyl-<small>D</small>-amino acid and H<sub>2</sub>O, whereas its two products are acid and <small>D</small>-amino acid.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N-acyl-<small>D</small>-amino acid amidohydrolase. It employs one cofactor, zinc.
As of late 2007, 8 structures have been solved for this class of enzymes, with PDB accession codes , , , , , , , and .