In enzymology, a N-acetylornithine carbamoyltransferase () is an enzyme that catalyzes the chemical reaction:
The enzyme converts N(2)-acetyl-L-ornithine to N-acetyl-L-citrulline by transferring a carbamoyl group from carbamoyl phosphate.
This enzyme belongs to the family of transferases that transfer one-carbon groups, specifically the carboxy- and carbamoyltransferases. The systematic name of this enzyme class is carbamoyl-phosphate:N2-acetyl-L-ornithine carbamoyltransferase. Other names in common use include acetylornithine transcarbamylase, N-acetylornithine transcarbamylase, AOTC, and carbamoyl-phosphate:2-N-acetyl-L-ornithine carbamoyltransferase.
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes , , , and .