In enzymology, a N-acetyldiaminopimelate deacetylase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are N-acetyl-LL-2,6-diaminoheptanedioate and H<sub>2</sub>O, whereas its two products are acetate and LL-2,6-diaminoheptanedioate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N6-acetyl-LL-2,6-diaminoheptanedioate amidohydrolase. Other names in common use include N-acetyl-L-diaminopimelic acid deacylase, N-acetyl-LL-diaminopimelate deacylase, and 6-N-acetyl-LL-2,6-diaminoheptanedioate amidohydrolase. This enzyme participates in lysine biosynthesis.