Mycodrosophila is a genus of vinegar flies, insects in the family Drosophilidae. There are at least 120 described species in Mycodrosophila.
It belongs to the monophyletic Zygothrica genus group consisting of the mycophagous genera Hirtodrosophila, Mycodrosophila, Paramycodrosophila, and Zygothrica. It is itself monophyletic.