Mister Venezuela 2000 was the fifth Mister Venezuela pageant. It was held at the Estudio 1 de Venevisión in Caracas, Venezuela on November 22, 2000.
At the end of the event, Alejandro Otero of Distrito Federal titled Luis Nery of PenÃÂnsula Goajira as Mister Venezuela 2000. He represented Venezuela at the Manhunt International 2001 pageant placing as 2nd runner-up.
The runner-up position went to Cristóbal Lander of Miranda.
26 contestants competed for the title.