Interstate 310 (I-310), also designated as Mississippi Highway 601 (MS 601), was a proposed auxiliary route of I-10 in Gulfport, Mississippi, which had been planned to connect US Highway 90 (US 90) near the Port of Gulfport to I-10.
In 2021, the route for I-310 was identified within High Priority Corridors 93(B) and 100(B) as part of the development of proposed I-14. High Priority Corridor 94 connects with this route's proposed northern terminus along I-10 and continues northward, connecting to I-59 in Hattiesburg, Mississippi and running parallel to US Highway 49 for its entire length.
The original corridor for I-310 began at a new intersection near US Highway 90 in Gulfport that would have facilitated shipping traffic from the Port of Gulfport. I-310 north of this intersection would have been elevated over the city, and a partial exit was to be constructed at 28th Street allowing for exiting southbound and entering northbound traffic. I-310 would have then continued north-west across wetlands and forests before terminating at Exit 31 (Canal Road) of I-10.
Highway planning began in 1994 with a meeting between the Mississippi Department of Transportation (MDOT) and leaders for Harrison County, Mississippi, Gulfport, and the Port of Gulfport. All parties agreed to the development of a new auxiliary road connecting the port and I-10, and the Mississippi State Legislature approved of the project in 1996. Right-of-way acquisitions began in 2006. Preliminary land-clearing began on the section between I-10 and 28th Street in 2009 after the U.S. Army Corps of Engineers issued a conservation easement. This permit would be invalidated in 2011, bringing construction of the new I-310 to a stop.
In 1999, MDOT proposed an elevated freeway for the portions of I-310 in downtown Gulfport. This plan was rejected by city officials and business leaders, and MDOT shelved plans for the I-310 corridor south of 28th Street indefinitely in 2007.
The Infrastructure Investment and Jobs Act of 2021 designated an extended future I-14 corridor that would encompass the original "14th Amendment Highway" and "Gulf Coast Strategic Highway" concepts. Future I-310/MS 601 became part of High Priority Corridor 93(b), the South Mississippi Corridor, and High Priority Corridor 100(B), the Central Mississippi Corridor.