Methanofollis is a genus of archaean in the family Methanomicrobiaceae.
Methanofollis ("a methane-producing bag") is a non-motile, Gram-negative, obligately anaerobic, mesophilic archaeon that produces methane. It grows between the temperatures 20–45 ðC (optimum 34–40 ðC), and at the pH of around 7.
The genome of the archaeon has not yet sequenced. The G + C content of the DNA is determined to be 60.0%.
The cells of Methanofollis are highly irregular cocci, with diameter of 1.25–2.0 üm. The major polar lipids are phospholipids, glycolipids, and phosphoglycolipids. It utilizes H<sub>2</sub>/CO<sub>2</sub>, formate, 2-propanol/CO<sub>2</sub>, and 2-butanol/CO<sub>2</sub> for growth and methanogenesis. No growth has been observed on acetate, trimethylamine, methanol, ethanol, 2-propanol, isobutanol, or 2-butanol as catabolic substrates.
Most species of the archaeon are isolated from anaerobic high-rate wastewater bioreactors or solfataric fields. For example, M. tationis was isolated from a solfataric field on Mount Tatio in the Atacama desert in northern Chile.
The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI).