Mauchline railway station was a railway station serving the town of Mauchline, East Ayrshire, Scotland. The station was originally part of the Glasgow, Paisley, Kilmarnock and Ayr Railway.
The station opened on 9 August 1848. It was renamed Mauchline for Catrine by 1887, and renamed back to Mauchline in 1903. The station closed to passengers on 6 December 1965.
Today this line is still open as part of the Glasgow South Western Line.
The Garrochburn Goods Depot lay a few miles to the north and until circa 1926 a Mossgiel Tunnel Platform was located just to the north of the tunnel mouth on the Hurlford side.