Matyshevo () is a rural locality (a selo) and the administrative center of Matyshevskoye Rural Settlement, Rudnyansky District, Volgograd Oblast, Russia. The population was 1,523 as of 2010. There are 14 streets.
Matyshevo is located 32 km west of Rudnya (the district's administrative centre) by road. Matyshevo (settlement) is the nearest rural locality.