In enzymology, a mandelamide amidase () is an enzyme that catalyzes the chemical reaction
Thus, the two substrates of this enzyme are (R)-mandelamide and H<sub>2</sub>O, whereas its two products are (R)-mandelate and NH<sub>3</sub>.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is mandelamide hydrolase. This enzyme is also called Pseudomonas mandelamide hydrolase.