The Macchi MB.320 was an Italian cabin monoplane designed and built by Macchi. Only a small number were built.
The MB.320 was built using experience gained from the company's previous aircraft, the MB.308. The MB.320 was a low-wing cabin monoplane with retractable tricycle landing gear and powered by two wing-mounted Continental E185 engines. It had room for a pilot and five passengers.
The prototype MB.320, registered I-RAIA, was first flown on 20 May 1949. The aircraft flew well, but was expensive to buy with only a small domestic market for the type and only a small number were exported. Three aircraft were sold to East African Airways for use as feederliners.