Lin-ay sang Negros 2016, the 22nd edition of the annual Lin-ay sang Negros pageant was held on April 18, 2016 at the Pana-ad Stadium. A total of 11 cities and 12 municipalities sent their representatives. Lin-ay sang Negros 2015 winner Jessica Zevenbergen of San Carlos City, crowned her successor Alyssa Jimenea of Victorias at the end of the event. This is the first time the pageant is held with the province no longer a part of Western Visayas but the Negros Island Region.