Lilita Zatlere (born 24 February 1953 Riga) is a Latvian businesswoman. She served as the First Lady of Latvia from 2007 to 2011 during the presidency of her husband, Valdis Zatlers.
Zatlere was born in Riga on 24 February 1953. She attended elementary school in Rëga No. 15 Eight-Year School. She then studied at Rëga Trade Technical School and the Soviet-era People's University. After completing school, Zatlere began working with art at an antiques shop.
Following the restoration of Latvian independence, Lilita Zatlere was appointed to a Riga city commission which focused on compensating Latvians who had lost property during the Soviet occupation. She served on the committee for three years. From 1995 to 2007, she was the director of the Private Orthopaedic Practice, which supplied spinal implants to the , which was headed by her second husband, Valdis Zatlers.