In enzymology, L-sorbose oxidase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are L-sorbose and oxygen. Its products are 5-dehydro-D-fructose and hydrogen peroxide.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is L-sorbose:oxygen 5-oxidoreductase.