In enzymology, L-rhamnose 1-dehydrogenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are L-rhamnofuranose and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are L-rhamnono-1,4-lactone, reduced NADH, and a proton.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD<sup>+</sup> or NADP<sup>+</sup> as acceptor. The systematic name of this enzyme class is L-rhamnofuranose:NAD<sup>+</sup> 1-oxidoreductase. This enzyme participates in fructose and mannose metabolism.