L-pipecolate oxidase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are L-pipecolic acid and oxygen. Its products are (2S)-2,3,4,5-tetrahydropyridine-2-carboxylic acid and hydrogen peroxide.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH group of donors with oxygen as acceptor. The systematic name of this enzyme class is L-pipecolate:oxygen 1,6-oxidoreductase. Other names in common use include pipecolate oxidase, and L-pipecolic acid oxidase.
In water, the product is hydrolysed to L-allysine, which can be converted to L-lysine in the yeasts such as Rhodotorula glutinis in which the enzyme is found.