In enzymology, L-idonate 5-dehydrogenase () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are L-idonic acid and oxidised nicotinamide adenine dinucleotide (NAD<sup>+</sup>). Its products are 5-oxo-D-gluconic acid, reduced NADH, and a proton. The enzyme can also use the alternative cofactor, nicotinamide adenine dinucleotide phosphate.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD<sup>+</sup> or NADP<sup>+</sup> as acceptor. The systematic name of this enzyme class is L-idonate:NAD(P)<sup>+</sup> oxidoreductase.