In enzymology, L-glutamate oxidase () is an enzyme that catalyzes the chemical reaction
The three substrates of this enzyme are L-glutamic acid, water, and oxygen. Its products are ñ-ketoglutaric acid, hydrogen peroxide, and ammonia.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH<sub>2</sub> group of donors with oxygen as acceptor. The systematic name of this enzyme class is L-glutamate:oxygen oxidoreductase (deaminating). Other names in common use include glutamate (acceptor) dehydrogenase, glutamate oxidase, glutamic acid oxidase, glutamic dehydrogenase (acceptor), and L-glutamic acid oxidase. It employs one cofactor, FAD.