L'Ascension-de-Notre-Seigneur () is a parish municipality in Quebec, Canada, located within the regional county municipality of Lac-Saint-Jean-Est in the Saguenay-Lac-Saint-Jean region. Its name is French for "the Ascension of Our Lord".
The municipality had a population of 2,079 in the 2021 Canadian Census.
The area opened for colonization in 1896, and the parish was formed on June 8, 1916. In 1919, the place was incorporated, taking the parish's name.
In the 2021 Census of Population conducted by Statistics Canada, L'Ascension-de-Notre-Seigneur had a population of living in of its total private dwellings, a change of from its 2016 population of . With a land area of , it had a population density of in 2021.
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