Kotwali Thana () is a thana of Khulna Metropolitan Police in the Division of Khulna, Bangladesh. It is also known as Khulna Sadar. It is the most densely populated subdistrict in the division.
Kotwali Thana is located at . It has 36895 households and total area 9.45 km<sup>2</sup>.
According to the 2022 Bangladeshi census, Kotwali Thana had 65,425 households and a population of 248,220. 7.23% were under 5 years of age. Kotwali had a literacy rate of 88.40%: 90.40% for males and 86.32% for females, and a sex ratio of 103.80 males per 100 females.
At the 1991 Bangladesh census, Kotwali Thana had a population of 191,910, of whom 111,725 were aged 18 or older. Males constituted 54.87% of the population, and females constituted 45.13%. It had an average literacy rate of 59.7% (7+ years), against the national average of 32.4%. At the 2001 census, it had a population of 250,651, and at the 2011 Census of Bangladesh, 224,444.