The Knoxville and Holston River Railroad operates over within Knoxville and Marbledale, Tennessee. This short line railroad was created in 1998 and is currently owned by Gulf and Ohio Railways. The railroad also hosts a tourist train run by Gulf & Ohio Railways, the Three Rivers Rambler.
KXRR currently serves 13 customers with the majority of them at the Forks of the River industrial park.
KXRR recently ended operations on its K-Line which extended almost four miles from a connection with Norfolk Southern to an asphalt plant on the Tennessee River which it served. The line is being donated by the railroad to the City of Knoxville to use as a trail. The K-Line included a tunnel.