Kholosa Mthikazi Biyana (born 6 September 1994) is a South African soccer player and youth coach who plays as a midfielder for SAFA Women's League club Mamelodi Sundowns Ladies and the South Africa women's national team.
She was part of the national team when they won their maiden continental title at the 2022 Women's Africa Cup of Nations.
In 2023, she won her first club continental title with Sundowns Ladies at the 2023 CAF Women's Champions League.
Biyana participated in three FISU World University Games.
She was born in Ngcobo. She began playing soccer when she was 8.
Biyana captained UKZN Ladies in their maiden Women's Varsity Football season in 2018.
In 2020 she signed for Segunda Federación side Sporting de Gijón.
She signed for UWC Ladies at the start of the 2023 season.
In 2023, she joined SAFA Women's League side Mamelodi Sundowns Ladies.
She won the 2023 CAF Women's Champions League, 2023 COSAFA Women's League and the 2023 Hollywoodbets Super league titles with Sundowns.
Biyana started for all three matches played by South Africa at the 2019 FIFA Women's World Cup. She was part of the South Africa women's national team that won the 2022 Women's Africa Cup of Nations. She was on the team that made it to the Round of 16 at the 2023 FIFA Women's World Cup and started for 2 of South Africa's 4 matches.
In 2024 she joined the Mamelodi Sundowns Ladies Academy as an assistant coach. In 2025 she was appointed head coach of the under-17 team that went on to win the Gauteng Women's Development League and qualify for the 2025 CAF U-17 Girls Integrated Football Tournament -COSAFA region. Her team won the tournament scoring 30 goals and only conceding once.
South Africa
Mamelodi Sundowns Ladies
Mamelodi Sundowns Ladies Academy