K. S. Jayalakshmi is an Indian actress who works in the Tamil film and television industries. She has acted in comedy roles in movies. Jayalakshmi made her acting debut in a Kannada film before acting in the Tamil film Etharkum Thuninthavan released in 1976.
She has since then been featured in more than a hundred films, including Agni Satchi, Poikkal Kudhirai, Manathil Uruthi Vendum, Guru Sishyan, Pudhu Pudhu Arthangal and Kaadhale Nimmadhi.
She was regularly cast in productions by Kavithalaya Productions and has acted in numerous films by K. Balachander.