Juupajoki is a municipality of Finland. It is located in the Pirkanmaa region. The municipality has a population of (), which makes it the least populous municipality in Pirkanmaa. It covers an area of of which is water. The population density is . Korkeakoski is the administrative center of the municipality.
Neighbouring municipalities are Jämsä, Mänttä-Vilppula, Orivesi and Ruovesi. The city of Tampere is located southwest of Juupajoki.
The municipality is unilingually Finnish.