John Mayhew may refer to:
- John Mayhew (musician) (1947âÂÂ2009), drummer for the progressive rock band Genesis
- John Mayhew (cricketer) (1909âÂÂ1999), English cricketer
- John W. Mayhew (1885âÂÂ1941), former head football coach at Louisiana State University
- John Mayhew (Conservative politician) (1884âÂÂ1954), British politician, MP for East Ham North, 1931âÂÂ1945
- John Mayhew, 18th-century London cabinet-maker, see Ince and Mayhew
- John Mayhew (doctor) (1954âÂÂ2025), New Zealand sports physician, North Harbour team doctor formerly chief medical official to the New Zealand national rugby union team