Jesse and Ira Tuthill House is a historic home located at Mattituck in Suffolk County, New York. It was built in two stages, 1799 and 1841. The original two-room house was incorporated as a -story wing for the larger 2-story, nine-room house. The final 1841 house is a -story residence with a modestly pitched gable roof with a wide frieze running beneath the roof eave.
It was added to the National Register of Historic Places in 2006.
The Mattituck-Laurel Historical Society owns the 1799 Jesse Tuthill House and 1841 Ira Tuthill House and operates them as period historic house museums.