Jan Holub (born 7 August 1991) is a Czech motorcycle speedway rider. He rode for Kolejarz Opole during the Polish Second League.
Jan comes from a 3-generation speedway family; his grandfather (Jan I) and father (Jan II) were also speedway riders with high records. Jan I finished sixth in the 1972 Speedway World Pairs Championship, and Jan II participated in 1986 and 1989 Under-21 World Championship.