Jan Holub (born May 3, 1983, in Liberec, Czechoslovakia) is a former Czech professional ice hockey defenceman who played in the Czech Extraliga.
Holub has spent his whole career with HC Liberec, beginning at junior-level. He was picked for the 2001 NHL Entry Draft by the New York Islanders as a seventh-round pick, but has never played for the Islanders. He represented the Czech Republic junior team in 2003 World Junior Ice Hockey Championships, when Czech team was in quarterfinals.