Jamesville Colony is a census-designated place (CDP) and Hutterite colony in Yankton County, South Dakota, United States. The population was 110 at the 2020 census. It was first listed as a CDP prior to the 2020 census.
It is in the northwest part of the county, on the north side of the James River. It is north of Utica and north-northwest of Yankton, the county seat.
It is in the Menno School District 33-2.