James U. Gillespie (died June 20, 1981), also known as J. U. Gillespie and John U. Gillespie, was an American politician. He served as a Democratic member of the Florida House of Representatives.
Gillespie was a civic leader.
Gillespie served in the Florida House of Representatives from 1939 to 1941.
Gillespie died on June 20, 1981, in New Smyrna Beach, Florida, at the age of 89.