James Leslie (c.1610–1678) was a Scottish physician who was principal of Marischal College (now part of Aberdeen University) from 1661 to 1678.
He was born in Aberdeen the son of Thomas Leslie a burgess. He studied medicine at Aberdeen University and qualified as a physician. In 1661 he replaced William Moir as principal of Marischal College.
He died in 1678. His position as principal was filled by Robert Paterson.
He married three times:
Firstly to Elizabeth Lumsden of Ruthrieston (d.1663) and had three children:
Secondly he married Miss Gray, daughter of Provost Gray of Aberdeen and had three more children:
Lastly he married Margaret Bennet.