James Johnstone may refer to:
- James Johnstone, 1st Earl of Hartfell (1602âÂÂ1653), Scottish peer and royalist
- James Johnstone, 2nd Marquess of Annandale (c. 1687âÂÂ1730), Scottish peer
- Sir James Johnstone, 3rd Baronet (1697âÂÂ1772), Scottish MP for Dumfries Burghs 1743âÂÂ1754
- Sir James Johnstone, 4th Baronet (1726âÂÂ1794), Scottish MP for Dumfries Burghs 1784âÂÂ1790 and Weymouth & Melcombe Regis
- James Johnstone (explorer) (1759âÂÂ1823), master, later lieutenant, aboard HMS Chatham during George Vancouver's 1791âÂÂ95 expedition
- James Hope-Johnstone, 3rd Earl of Hopetoun (1741âÂÂ1816), Scottish representative peer and Lord Lieutenant of Linlithgowshire
- Chevalier de Johnstone (1719âÂÂc. 1791), actually James Johnstone, Jacobite army officer
- James Johnstone (1801âÂÂ1888), Scottish MP for Clackmannanshire & Kinross-shire
- James Johnstone (publisher) (1815âÂÂ1878), British newspaper proprietor
- James Johnstone (biologist) (1870âÂÂ1932), British biologist and oceanographer
- James Johnstone (stock breeder) (1859âÂÂ1933), New Zealand businessman and stock breeder
- Jim Johnstone (poet) (born 1978), Canadian physiologist and poet
- Jim Johnstone (umpire) (1872âÂÂ1927), American baseball umpire
- Jim Johnstone (basketball) (born 1960), American basketball player
- Jimmy Johnstone (1944âÂÂ2006), Scottish footballer
- James Johnstone (rugby union) (born 1990), Scottish rugby union player
See also