Jacobabad Junction Railway Station (, ) is located in Jacobabad, Sindh, Pakistan. Jacobabad serves as a major junction for Pakistan Railways network with tracks branching off to Kot Addu (via Dera Ghazi Khan). The station is staffed and has a booking office.
In 1906, it was converted into a junction.
The routes are Jacobabad from linked with Lahore, Karachi, Peshawar, Rawalpindi, Quetta, Multan, Faisalabad, Hyderabad, Rohri, Sukkur, Bahawalpur, Dera Ghazi Khan, Kot Adu, Gujrat, Larkana, Gujranwala, Khanewal, Nawabshah, Attock, Dadu, and Nowshera.