Interferon alpha-2 is a protein that in humans is encoded by the IFNA2 gene.
Human interferon alpha-2 (IFNñ2) is a cytokine belonging to the family of type I IFNs. IFNñ2 is a protein secreted by cells infected by a virus and acting on other cells to inhibit viral infection. The first description of IFNs as a cellular agent interfering with viral replication was made by Alick Isaacs and Jean Lindenmann in 1957. The history of this finding was recently reviewed. There are 3 types of IFNs: Interferon type I, Interferon type II and Interferon type III. The type II IFN, also called IFNó, is produced by specific cells of the immune system. Unlike type I and type III IFNs, IFNó has only a modest role in directly restricting viral infections. Type I and type III IFNs act similarly. However, the action of type III IFNs, also known as IFNû, is limited to epithelial cells while type I IFNs act on all body's cells.
Type I IFNs form a family of several proteins: in humans, there are 13 ñ subtypes, 1 ò subtype, 1 àsubtype and other less studied subtypes (ú and õ). IFNñ2 was the first subtype to be characterized in the early eighties. As a result, IFNñ2 was widely used in basic research to elucidate biological activities, structure and mechanism of action of type I IFNs. IFNñ2 was also the first IFN to be produced by the pharmaceutical industry for use as a drug. Thereby, IFNñ2 is the best known type I IFN subtype. The properties of IFNñ2 are widely shared by the other type I IFNs, although subtle differences exist.
The gene encoding IFNñ2, the IFNA2 gene, is clustered with all other type I IFN genes on chromosome 9 and as all type I IFN genes, it is devoid of intron. The open reading frame (coding sequence) of IFNA2 codes for a pre-protein of 188 amino acids with a 23 amino acid signal peptide allowing secretion of the mature protein. The mature protein is made of 165 amino acids, one less than the other human IFNñ subtypes. The secondary structure of IFNñ2 consists of five ñ-helices: A to E, from the N-terminal to the C-terminal end. Helices A, B, C and E are organized as a bundle with a long loop between the helices A and B (the A-B loop) and two disulfide bonds which connect helix E to the A-B loop and helix C to the N-terminal end. Several variants, or allelic variants, have been identified in the human population. Among them, IFNñ2a and IFNñ2b are better known by their commercial name, Roferon-A and Intron A, respectively. Upstream of the coding sequence is the promoter region that contains sequences that regulate the transcription of the IFNA2 gene into a messenger RNA (mRNA). The amino acid sequences of IFNñ2a and IFNñ2b differ only at position 23 (lysine in IFNñ2a, arginine in IFNñ2b).
When a cell is infected by a virus, some components of the virus, mainly viral nucleic acids, are recognized by specialized cellular molecules such as RIG-I, MDA5 and some toll-like receptors (TLR). This recognition induces the activation of specific serine kinases, enzymes which activate by phosphorylation the IFN regulatory factors (IRF), IRF3 and IRF7. IRF3 and IRF7 are themselves transcription factors that translocate into the nucleus and activate the transcription of type I IFNs genes and thereby initiate the process leading to the secretion of IFN by the infected cells. The "danger" signals carried by viruses were the first IFN inducers described but it is now known that non-viral "danger" signals, such as some types of dead cells, can stimulate the synthesis of type I IFNs.
Induced IFNñ2 is secreted by the infected cells and acts locally as well as systemically on cells expressing a specific cell surface receptor able to bind type I IFNs. The type I IFN receptor (IFNAR) is composed of two subunits, IFNAR 1 and IFNAR 2, which are expressed by all body's cells. After binding to its receptor, type I IFNs activate multiple cellular factors that transduce the signal from the cell surface into the nucleus. The main signaling pathway activated by type I IFNs consists of a series of events:
ISGs encode proteins that modulate cellular functions. Following viral infection, many ISGs lead to the inhibition of the viral spread. Several ISGs inhibit viral replication in the infected cells. Other ISGs protect neighbouring uninfected cells from being infected by inhibiting viral entry. Several hundreds of ISGs are known to be activated by type I IFNs and are listed in a searchable database named interferome (http://www.interferome.org/).
The broad spectrum of ISGs explains the wide range of biological activity of type I IFNs. In addition to their antiviral activity, type I IFNs also inhibit the proliferation of cells and regulate the activation of the immune system.
Type I IFNs exert potent antitumor activity by several mechanisms such as:
Type I IFNs can have detrimental effects during viral and non-viral infections (bacterial, parasitic, fungal). This is due in part by the ability of type I IFNs to polarize the immune system towards a specific type of response in order to interfere with virus infections.
When improperly regulated, IFN production or IFN-induced signalling can result in autoimmune diseases, such as systemic lupus erythematosus.
If given orally, IFNñ2 is degraded by digestive enzymes and is no longer active. Thus, IFNñ2 is mainly administrated by injection essentially subcutaneous or intramuscular. Once in the blood, IFNñ2 is rapidly eliminated by the kidney. Due to the short life of IFNñ2 in the organism, several injections per week are required. Peginterferon alpha-2a and Peginterferon alpha-2b (polyethylene glycol linked to IFNñ2) are long-lasting IFNñ2 formulations, which enable a single injection per week.
Recombinant IFNñ2 (ñ2a and ñ2b) has demonstrated efficiency in the treatment of patients diagnosed with some viral infections (such as chronic viral hepatitis B and hepatitis C) or some kinds of cancer (melanoma, renal cell carcinoma and various hematological malignancies). Yet, patients on therapy with IFNñ2 suffer from adverse effects which often require to reduce or even stop the treatment. These adverse effects include flu-like symptoms such as chills, fever, joint and muscle pain, depression with suicidal ideation, and a reduction in the number of blood cells. Thereby, IFNñ2 has been progressively replaced by better tolerated drugs, such as antiviral agents or targeted antitumor therapies. Chronic viral hepatitis C is the main indication for which IFNñ2 remains widely used. Nevertheless, there is increasing evidence that endogenous type I IFNs plays a role in the induction of an immune antiviral response and that they can enhance the antitumor activity of chemotherapies, radiotherapies and some targeted therapies. Therefore, an important future goal for scientists is to modify IFNñ2 in order to obtain an active molecule to be used in the clinic that does not exert adverse effects. Anecdotal evidence suggests interferon alfa 2b is effective antiviral treatment in COVID-19