The Humboldt big-eared brown bat (Histiotus humboldti) is a species of vesper bat in the family Vespertilionidae. It is found in Colombia and Venezuela.
Histiotus humboldti is distributed in the subtropical forests of the eastern foothills of the mountains outside the Ecuadorian Andes, between in the eastern subtropical zoogeographical floor, unlike Histiotus montanus which is recorded at higher altitudes of .