Hoseyn Khan Shamlu, also Husain Khan Shamlu (; died 1535), son of ÿAbdi Beg à  ÃÂmlu, was a member of the Turkoman Shamlu tribe, and a Safavid governor of Herat from 1524âÂÂ25. He was nephew of Shah Ismail I, his father Abdi Beg Shamlu having married a sister of the Shah. He was also a brother of Durmeà ¡ Khan à  ÃÂmlu, whom he replaced as Governor of Herat upon his death.
Hoseyn Khan Shamlu was the Lala (guardian) of the young prince Sam Mirza. He married one of his daughters to him. They had a daughter who was married to the Georgian ruler Prince Jesse of Kakheti.
In 1525âÂÂ26 and in 1526âÂÂ27, Hoseyn Khan Shamlu resisted the offensive of the Uzbeks against Herat, standing a siege of seven months, before he was relieved by Imperial troops. However, in 1529, he was forced to surrender Herat to the Uzbek Obayd Khan. Both Hoseyn Khan Shamlu and Sam Mirza fled Herat at that time.
In 1533, Shah Tahmasp I executed Hoseyn Khan Shamlu on suspicions that he was trying to put Sam Mirza on the throne.
Hoseyn Khan Shamlu was likely portraited in two miniatures of the Cartier Hafiz painted by Shaykhzada in Herat circa 1527: the ', and the now-lost '. Hoseyn Khan Shamlu was Shaykhzada' patron, and he is depicted in these two miniatures as a mature man with a full mustache.