Holmesville is a census-designated place (CDP) in Gage County, Nebraska, United States. As of the 2020 census, Holmesville had a population of 60.
Holmesville got its start in the year 1880, following construction of the railroad through the territory. It was named for its postmaster, Morgan L. Holmes.
Holmesville is in central Gage County, on the east side of the Big Blue River, a south-flowing tributary of the Kansas River. The community is southeast of Beatrice, the Gage County seat.
According to the U.S. Census Bureau, the Holmesville CDP has an area of , all land.