Holbrook Creek is a creek in east central Yukon, Canada.
The landscape surrounding Holbrook Creek lies in the Fort Selkirk Volcanic Field of the Northern Cordilleran Volcanic Province. During the Pleistocene period, a basaltic lava flow from the Fort Selkirk field engulfed the mouth of the Holbrook Creek. Remnants of this lava flow are exposed along the Yukon River for about one kilometre.