Holbert Run is a long 2nd order tributary to the Ohio River in Hancock County, West Virginia. This is the only stream of this name in the United States.
Holbert Run rises about north of Sun Valley, West Virginia, in Hancock County and then flows generally west-southwest to join the Ohio River about north of Weirton.
Holbert Run drains of area, receives about of precipitation, has a wetness index of 279.73, and is about 87% forested.