Hesperonatalius harrietae is a species of mygalomorph spider in the Anamidae family. It is endemic to Australia. It was described in 2017 by Mark Castalanelli, Joel Huey, Mia Hillyer and Mark Harvey.
The species occurs in Western Australia in the Geraldton Sandplains bioregion. The type locality is Oakajee, 25 km north of Geraldton.