HMS Harlequin was a 16-gun built for the Royal Navy during the 1830s.
Harlequin had a length at the gundeck of and at the keel. She had a beam of , a draught of and a depth of hold of . The ship's tonnage was 432 <small></small> tons burthen. The Racer class was armed with a pair of 9-pounder (or 18-pounder) cannon and fourteen 32-pounder carronades. The ships had a crew of 120 officers and ratings.
Harlequin, the fifth ship of her name to serve in the Royal Navy, was ordered on 28 March 1832, laid down in November 1832 at Pembroke Dockyard, Wales, and launched on 18 March 1836. She was completed on 25 October at Plymouth Dockyard and commissioned on 16 August of the same year.