Gyllenstierna is a Danish and Swedish noble surname that may refer to:
- Christina Gyllenstierna (1494/5âÂÂ1559), wife of Sten Sture the Younger and Swedish resistance leader
- Ebbe Gyllenstierna (1911âÂÂ2003), Swedish Army officer and modern pentathlete
- Elisabeth Gyllenstierna (1581âÂÂ1646) Swedish court official
- Eric Gyllenstierna (1882âÂÂ1940), Swedish diplomat*Görwel Gyllenstierna (1646âÂÂ1708), Swedish noblewoman duellist
- Johan Göransson Gyllenstierna (1635âÂÂ1680), Swedish statesman
- Karin Gyllenstierna, 17th century Swedish courtier
- Maria Gustava Gyllenstierna (1672âÂÂ1737), Swedish countess, writer and translator
- Margareta Gyllenstierna (1689âÂÂ1740), Swedish political activist
- Nils Gyllenstierna (1530âÂÂ1601), Swedish statesman who was the Lord High Chancellor of Swedenfrom 1560 to 1590 and Lord High Steward of Sweden from 1590 to 1601
See also