Greek National Road 65 is a national highway of Greece. It connects the Greek National Road 2 at Diavata, a northwestern suburb of Thessaloniki, with Kilkis. Most of the National-Road has been upgraded to a Motor-Road, connecting Thessaloniki via Kilkis with Doirani, the eastern border of Greece and North Macedonia.
The Road will be constructed as a Motor-Road, the part Nea Santa until Kilkis are ready, the second part after Kilkis will be constructed until 2014.
Ministerial Decision G25871 of 9 July 1963 created the EO65 from the EO39, which existed by royal decree from 1955 until 1963, and followed the same route as the current EO65. In 1998, the Hellenic Statistical Authority extended the EO65 designation from Kilkis to Petritsi via Mouries, taking over some of the Kilkis regional unit's Provincial Roads.