National Road 27 (, abbreviated as EO27) is a single carriageway road in central Greece. It connects the Greek National Road 3 at Bralos, south of Lamia, with the GR-48 near Itea, passing through Amfissa. It is part of the European route E65. The road passes through the regional units Phocis and Phthiotis.
National Road 27 passes through the following places:
Ministerial Decision G25871 of 9 July 1963 created the EO27 from the old EO22, which existed by royal decree from 1955 until 1963, and followed the same route as the current EO27.