In enzymology, glycerol dehydrogenase (acceptor) () is an enzyme that catalyzes the chemical reaction
The two substrates of this enzyme are glycerol and an electron acceptor. Its products are dihydroxyacetone and the corresponding reduced acceptor.
This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with other acceptors. The systematic name of this enzyme class is glycerol:acceptor 1-oxidoreductase. This enzyme is also called glycerol:(acceptor) 1-oxidoreductase. It employs one cofactor, PQQ.