Gillespie Run is a long 2nd order tributary to Middle Wheeling Creek in Ohio County, West Virginia.
According to the Geographic Names Information System, it has also been known historically as:
Gillespie Run rises about 0.25 miles northwest of Beham, Pennsylvania, and then flows west-northwesterly to join Middle Wheeling Creek about 0.5 miles south of Camp Giscowhego.
Gillespie Run drains of area, receives about 41.3 in/year of precipitation, has a wetness index of 280.65, and is about 70% forested.