Gavkhuni-ye Jonubi Rural District () is in the Central District of Varzaneh County, Isfahan province, Iran. Its capital is the village of Oshkohran, whose population at the time of the 2016 National Census was 1,463 people in 417 households.
In 2021, Bon Rud District was separated from Isfahan County in the establishment of Varzaneh County and renamed the Central District. Gavkhuni-ye Jonubi Rural District was created in the district.