The enzyme GDP-glucosidase () catalyzes the chemical reaction
Thus, the two substrates of this enzyme are GDP-glucose and H<sub>2</sub>O, whereas its two products are <small>D</small>-glucose and GDP.
This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is GDP-glucose glucohydrolase. Other names in common use include guanosine diphosphoglucosidase, and guanosine diphosphate <small>D</small>-glucose glucohydrolase.